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regulation of glucose and fatty oxidativemetabolism in animal
models. The effects of GDF15 on metabolism have similarities
with the muscle fiber-derived or cytokines or peptides called
as
“
mytokine
”
. However, the study whether exercise have
effects on the plasma level of GDF15 has not been reported.
Therefore, we examined the change of GDF15 level with
metabolic parameters, including glucose and various kinds
of cholesterol after regular exercise.
Methods:
We enrolled the 20 subjects to be able to do aerobic
and strength exercise at least 3 times a week during 12 weeks.
All subjects had baseline records, including age, body mass
index (BMI), waist to hip ratio, body fat percentage. Blood
samples were collected the fasting, 2 and 4 hour after taking
the high calorie mixed meal at the starting and ending time
of study.
Results:
Two people excluded the study because of anemia and
laboratory errors. Total 18 subjects were enrolled and women
with average age of 37 ± 10 years. 4 subjects were glucose
intolerance (22.2%). 7 subjects had dyslipidemia (38.9%). Body
weight, BMI, waist to hip ratio, and body fat percentage had no
difference. Metabolic parameters in fasting state, including
glucose, insulin, cholesterol, HOMA-IR, and HOMA-
β
had no
difference. We investigated the differentials between fasting
and 2 hr postprandial glucose level (2 hr postprandial glucose
–
fasting glucose,
Δ
Glu 2-0) and between fasting and 2 hr
postprandial GDF15 level (2 hr postprandial GDF15
–
fasting
GDF15,
Δ
GDF 2-0) in subgroup according to glucose intoler-
ance.
Δ
Glu 2-0 showed significantly higher variation in
Subjects with glucose intolerance than in subjects with
normal glucose tolerance at time before exercises. However,
two groups showed no difference in
Δ
Glu 2-0 at time after
exercise.
Δ
GDF 2-0 in subjects with normal glucose tolerance
showed positive values at all of times before and after exercise.
However, in subjects with glucose intolerance showed nega-
tive
Δ
GDF 2-0 at times before exercise, but positive
Δ
GDF 2-0 at
times before exercise.
Conclusion:
Our data suggested that exercise in subjects with
glucose intolerance could result in recovery of GDF15 response
at postprandial times with improvement of postprandial
glucose level.
PA-04
Association between serum white blood cell counts and
hemoglobin A1c in a Korean adult population
DongJun KIM
1
*, JaeWon HONG
1
, JungHyun NOH
1
,
JongChol WON
2
, KyungSoo KO
2
.
1
Inje University Ilsanpaik
Hospital Department of Internal Medicine,
2
Inje University Ilsanpaik
Hospital Department of Internal Medicine, Cariovascular and
Metabolic Disease Center, Korea
Few studies have investigated the clinical effect of subclinical
inflammation on the use of the hemoglobin A1c (HbA1c) as a
screening parameter for diabetes or prediabetes. We investi-
gated the association between serum white blood cell counts
(WBC) within normal ranges as a marker of subclinical
inflammation and HbA1c levels in a Korean adult population.
Among 11,472 adults (>/=19 years old) who participated in the
2011
–
2012 Korea National Health and Nutrition Examination
Survey, participants with anti-diabetes/anti-hypertensive/
anti-lipid drugs, or history of previous cancer, or the presence
of chronic kidney disease, or positivity of HBsAg, or abnormal
WBC (<4000 micro/L or >10000 micro/L) were excluded. Finally
in 7116 participants, we investigated the association between
quartile (Q) of WBC and HbA1c. After adjusting for age, sex,
fasting plasma glucose (FPG), the presence of college gradu-
ation, smoking history, the presence of hypertension, serum
total cholesterol, serum triglyceride, and the presence of
anemia, the level of HbA1c increased with increase of WBC
(HbA1c, mean+/
−
SEM, 5.55+/
−
0.01% in Q1, 5.58+/
−
0.01% in Q2,
5.60+/
−
0.01% in Q3, and 5.65+/
−
0.01 in Q4, p < 0.001). After
adjusting for above mentioned covariates, the proportions (%)
of an HbA1c level of >/=5.7%, and >/=6.5% were also higher in
participants with the increase of WBC (proportion of >/=5.7%,
30.9+/
−
1.0% in Q1, 34.1+/
−
1.0% in Q2, 36.6+/
−
1.0% in Q3, and
39.3+/
−
1.0% in Q4, p < 0.001; proportion of >/=6.5%, 3.1+/
−
0.4%
in Q1, 2.9+/
−
0.4% in Q2, 4.1+/
−
0.4% in Q3, and 4.1+/
−
0.4% in Q4,
p = 0.022). In logistic regression analyses with above men-
tioned variables as covariates, the proportions of an HbA1c
level of >/=5.7%, and >/=6.5% increased with increase of WBC
(odds ratio [95% CI] for HbA1c level of >/=5.7%, 1.20 [1.02
–
1.42],
p = 0.028 in Q2, 1.37 [1.16
–
1.62], p < 0.001 in Q3, and 1.59 [1.35
–
1.89], p < 0.001 in Q4; for HbA1c level of >/=6.5%, 0.85 [0.45
–
1.63],
p = 0.632 in Q2, 1.79 [1.01
–
3.18], p = 0.048 in Q3, and 2.03 [1.13
–
3.64], p = 0.018 in Q4). Our study showed that serumWBC count
within normal ranges, marker of subclinical inflammation, are
associated with the increase of HbA1c after adjusting for
several covariates including FPG. The data suggested that the
subclinical inflammation need to be considered before using
HbA1c as a screening test for prediabetes or diabetes.
PA-05
Incidental risk for diabetes according to the change level of
body mass index in Korean men
Mi Hae SEO
1
, Sung Keun PARK
2
, Jae-Hong JAE-HONG
3
*,
Chang-Mo OH
4
.
1
Department of Internal Medicine, Gumi Hospital,
Soon Chun Hyang University, School of Medicine,
2
1Total Healthcare
Center, Kangbuk Samsung Hospital, Sungkyunkwan University,
School of medicine,
3
Departments of Preventive Medicine, School of
Medicine, Kyung Hee University,
4
The Korea Central Cancer Registry,
National Cancer Center, Korea
Objective:
Although accumulated evidence implies that
change of body mass index (BMI) have deep relation with
diabetes, there is little information about longitudinal rela-
tionship between the BMI change and diabetes in Asian.
Therefore, this study was conducted to evaluate the incidental
risk of diabetes according to the change of body mass index
Research design and methods:
Cohort of 31,138 middle aged
nondiabetic Korean men was followed-up annually or bienni-
ally from 2005 to 2010. On the basis of BMI in 2005, study
participants were categorized into 5 groups according to their
change levels of BMI for follow-up period from the lowest
quintile to the highest quintile (1stquintile
–
5thquintile). The
incidence and adjusted hazard ratios (HRs) for diabetes were
evaluated in 5 quintiles.
Results:
During 120,785.3 person-years of follow-up, 1,687
(5.3%) incident cases of diabetes developed between 2006 and
2010 (quintile 1: 6.6%, quintile 2: 4.3%, quintile 3: 4.0%, quintile
4: 5.0%, quintile5: 7.2%). When quintile 3 was set as a reference,
in adjusted model, the adjusted hazard ratios for diabetes
showed the J-shaped relationship with the levels of BMI
change [quintile 1: 1.52 (1.27
–
1.82), quintile 2: 0.85 (0.70
–
1.04),
quintile 3: 1 (reference), quintile 4: 1.27 (1.05
–
1.53), quintile 5:
2.85 (2.39
–
3.40), respectively].
Conclusions:
In this study, J-shaped relationship pattern was
demonstrated between risk for diabetes and the change levels
of BMI. This finding indicates that the risk of diabetes can
paradoxically increase not only by decreased BMI and but also
by increased BMI in Korean men.
PA-06
Elevated circulating plasma miRNA27b-3p in type 2 diabetes
and reduced miRNA3195 in diabetic nephropathy
Rui HAN
1
, Xu LIU
3
, Dian-ping SONG
1
, Xiaochen LIU
2
,
Wei YANG
1
, Hsiang-fu KONG
3
*, Marie Chia-mi LIN
3
.
1
Department of Endocrinology, The first Affiliated hospital of
Kunming medical University, Yunnan, China;
2
Department of
Epidemiology, School of Public Health, Brown University, USA;
3
HF
Kung Academician Workstation, Kunming Medical University,
Chunrong Road, Yunnan,
4
School of Biomedical Sciences, The Chinese
University of Hong Kong, China
Poster Presentations / Diabetes Research and Clinical Practice 120S1 (2016) S65
–
S211
S66