Diabetes Research and Clinical Practice - Volume 120 - Supplement 1 - October 2016

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Results: 4 patients were positive for tTGIgA and 2 patients were
positive for tTGIgG. Among them, one man was positive for
both tTGIgA and tTGIgG. His DQB1 genotype was 02:01/03:02
and disease duration 20.5 years. The positive rate of either
tTGIgA or tTGIgGwas 5/354 (1.4%). DQB1 genotypes were 03:02/
03:02, 02:01/03:03, and 02:01/06:01 in the remaining 3 patients
positive for tTGIgA, The DQB1 genotype was 03:03/04:01 in the
other patient with tTGIgG positivity. No gastrointestinal
symptoms or signs were detectable clinically in the 5 patients.
Conclusion: The positive rate of tTGIgA was 1.4% in T1D
patients in Taiwan and the patients with positive tTGIgA
carried DQB1*02:01, *03:02, or both.
PB-33
The association of YKL-40 genetic polymorphisms with
coronary artery disease in Taiwan population and diabetes
subgroup
Ke Hsin TING 1,2*, Shun Fa YANG2, Po Hui WANG2. 1Division of
Cardiology, Department of Internal Medicine, Changhua Christian
Hospital, Yunlin Branch, Yunlin, 2Institute of Medicine, Chung Shan
Medical University, Taichung, Taiwan
Background: YKL-40, released by human activated macro-
phages, neutrophils and vascular smooth muscle cells, plays a
role in the pathogenesis of endothelial dysfunction, athero-
sclerosis and abnormal angiogenesis. However, the associ-
ation of single nucleotide polymorphisms (SNPs) of YKL-40
with coronary artery disease (CAD) has not been clear in the
Taiwan population and diabetes subgroup. Materials and
methods: Five hundred and seventy-six unrelated Taiwanese
patients (male 397, female 179), receiving coronary angiog-
raphy because of chest pain at Chung Shan Medical University
Hospital were recruited from April 2007 to March 2013.
The blood samples were obtained for the analysis of YKL-40
SNPs rs6691378, rs10399805, rs4950928, rs880633 using real
time PCR assay from CAD case group (373 patients) and non-
CAD control group (203 controls). Thereafter, we analyzed
the relationships among CAD, the related clinical features and
the distributions of genotype and allele of these SNPs. We
assessed the demographic characteristics and the odds ratio
between case group and control group. Additionally, we also
analyzed the YKL-40SNPs from the diabetes subgroup(226
cases).
Results: In the female population, the frequencies of YKL-40
rs6691378 with GA/AA genotype [P = 0.008, odds ratio
(OR) = 2.267] and rs10399805 with GA/AA genotype (P = 0.004,
OR = 2.421,) were higher, as compared to their wild GG
genotypes in CAD than non-CAD groups After multivariate
analysis for YKL-40 SNPs and clinical features in the female
group. In addition to, recent 24 hours severe angina and
elevated cardiac enzyme, YKL-40 SNP rs10399805 GA/AA
(P = 0.009, OR = 2.524, 95% confidence interval = 1.254–5.078)
was an independent factors for CAD.
In the female diabetes population, the frequency of YKL-40
rs10399805 with GA genotype was higher, as compared to GG
genotype in CAD female group than non-CAD female group
(56.8% vs 29.6%, OR = 2.930, p = 0.047). The frequency of YKL-40
SNP rs880633 with CC genotype was lower, as compared to TT
genotype in CAD group than non-CAD group [9.1% vs 25.9%,
odds ratio (OR) = 0.190, p = 0.034].
Conclusion: In the Taiwanese female, YKL-40 SNP rs6691378
(-1371G/A) with GA/AA genotype and SNP rs10399805 (-247G/
A) with GA/AA genotype were associated with CAD. Based on
multivariate analysis, YKL-40 SNP rs10399805 (-247G/A)
however was an only independent genetic factor for CAD in
the Taiwanese female. Besides, in the Taiwan female diabetes,
YKL-40 SNP rs10399805 (-247G/A) with GA genotype can
increase genetic susceptibility of CAD. Additionally, YKL-40
SNP rs880633 (+2950 T/C) with CC genotype can protect genetic
susceptibility of CAD.
PB-34
Facial flushing and blood pressure among Cambodians with
T2D
Khun TOUCH1. 1Cambodian Diabetes Association, Siem Reap,
Cambodia
Purpose: Facial flushing is a proxy for variants of genes that
encode enzymes that metabolize alcohol, and is common
among Asians. Themetabolic syndrome (MS) is a cluster of risk
factors for type 2 diabetes (T2D) and cardiovascular disease:
hypertension, hyperglycemia, abdominal obesity, and dysli-
pidemia. Data suggest that flushers have increased risk for MS.
Rates of MS and T2D are increasing in Cambodia. Yet little is
known about flushing in persons with extant T2D or from
Cambodia. Data in this regard may have implications for
increasing rates of MS and T2D in Cambodia and point to
potential opportunities for intervention. This study investi-
gated the relationships among flushing, glycemia, and blood
pressure among Cambodians with T2D.
Methods: CDA patients were invited to participate if they were
35–80 years old, T2D >=one year, not taking insulin, no
documented psychiatric disorder, and no documented long –
term diabetes complications. After informed consent, trained
clinic staff administered Khmer-language Alcohol Use
Disorders Identification Test – Consumption (AUDIT – C).
Flushing was assessed with the question, Response options
were “yes” (drinker/flusher), “no” (drinker/non – flusher), or ‘I
don’t drink’ (non – drinker). Trained staff, assessed biomarkers
and reviewed medications. SBP and DBP were each taken,
according to JNC8 guidelines. A1c wasmeasured. A glucometer
was used to determine glucose. Data were analyzed.
Results: Fifty-nine participants were M = 56.6 (SD = 9.4) years
old and 60% female, diabetes duration
M = 4.8 (SD = 3.6) years, A1c M = 9.2% (SD = 1.8%), glucose
M = 153.5 mg/dl (SD = 50.3), SBP M = 130.2 (SD = 14.1) and DBP
M = 82.7 (SD = 8.0) mmhg. Results remained significant after
controlling for antihypertensive use and AUDIT – C scores.
Non-drinkers and non– flushers did not differ fromeach other,
p = 0.61. There were no significant differences between groups
for SBP, glucose or A1c.
Discussion: Flushing was associated with higher DBP. Lack of
findings for glycemic variables may suggest that flushing is
associated with cardiovascular, rather than metabolic,
mechanisms of MS. Other data suggest that the risk of MS
among flushers rises with increasing alcohol consumption.
More research is needed to determinewhether alcohol actually
potentiates MS in flushers, or whether flushing is a marker for
a third, as yet unidentified risk for MS. Limitations include
small sample, cross – sectional design, and inclusion of only
select components of MS.
Conclusions: In Cambodians with type 2 diabetes, drinking
despite facial flushing is associated with higher DBP.
Diabetes Performance Measures: An
Update and Future Directions
PC-01
Demographic and clinical features of diabetes mellitus in
Tuvalu patients
Yu-Tze LIN1, Nese ITUASO-CONWAY2, Yi-Sun YANG 3,4,
Chien-Ning HUANG3,4*. 1Chung-Shan Medical University Hospital,
Department of Nursing, Taiwan; 2Princess Margaret Hospital,
Tuvalu; 3Chung-Shan Medical University Hospital, Department of
Endocrinology and Metabolism, 4Chung-Shan Medical University,
Institute of Medicine, Taiwan
Background: It is well recognized that diabetes is a major and
increasing public health problem worldwide. Of particular
Poster Presentations / Diabetes Research and Clinical Practice 120S1 (2016) S65–S211 S86