Diabetes Research and Clinical Practice - Volume 120 - Supplement 1 - October 2016

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Among them, GE is the most Significant one. The role of GE in
the adolescent should not be overlooked.
PC-12
Regulation of vascular BK channels in diabetes by Nrf2
signaling
Hon-Chi LEE 1*, Yong LI1, Xiao-LiWANG1, Tong LU1. 1Mayo Clinic,
United States of America
Background and objective: The nuclear factor E2-related
factor-2 (Nrf2) signaling pathway has emerged as a master
regulator of cellular redox status. The large conductance
calcium-activated potassium (BK) channels, which are major
determinants of vasodilation, are impaired in diabetes melli-
tus (DM) due to the downregulation of BK- β1 by reactive oxygen
species-dependent mechanisms. The goal of this study is to
test the hypothesis that Nrf2 signaling plays a central role in
the regulation of BK channel function in DM.
Methods and results: Studies were performed combining
cellular, molecular, vascular, and electrophysiological techni-
ques. In type 2 diabetic db/db mouse aorta, Nrf2 protein
expression was significantly reduced, associated with signifi-
cant downregulation of BK- β1 and heme oxygenase 1 (HO-1), a
known Nrf2 downstream target. Also, the muscle ring finger
protein 1 (MuRF1), a known E-3 ligase targeting BK- β1, was
significantly upregulated. These findings were reproduced
by knockdown of Nrf2 by siRNA in cultured human coronary
smooth muscle cells (HCSMC), whereas adenoviral transfer
of Nrf2 gene in these cells was associated with downregu-
lation of MuRF1 and upregulation of BK-β1 and HO-1
expression. Activation of Nrf2 by dimethyl fumarate in high
glucose-cultured HCSMCs or in diabetic db/db mouse coronary
arteries preserved BK-β1 expression shown byWestern blot, BK
channel activities using patch clamp recordings, and vascular
function assessed by shear stress-mediated vasodilation in
isolated mouse coronary arteries.
Conclusions: Expression of BK-β1 is closely regulated by Nrf2
through its effect on MuRF1, which regulates BK- β1 degrad-
ation, and vascular BK channel function can be restored by
activation of Nrf2. Nrf2 should be considered a novel thera-
peutic target in the treatment of diabetic vasculopathy.
PC-13
Essential oil from sudachi peal improves glucose and lipid
metabolism
Keisuke FUKUTA1, Licht MIYAMOTO1*, Rie TAKAHASHI 1,
Toshiaki TAMAKI 2, Yasumasa IKEDA2, Koichiro TSUCHIYA1.
1 Dept. of Medical Pharmacology, Inst. of Biomedical Sciences,
Tokushima University Graduate School, 2Dept. of Pharmacology, Inst.
of Biomedical Sciences, Tokushima University Graduate School, Japan
Background: According to the statement of the International
Diabetes Federation, the diabetes population continues to
grow around the world. The number of people associated with
diabetes reaches 415 million in 2015, and one of 11 people is
estimated to be a patient. It is predicted that it will increase to
642 million by 2040 if we do not take any effective measures.
Recently, the alternativemedicine such as supplemental foods
and aromatherapy has attracted our attention. Among them,
the mechanisms of the aromatherapy are still unknown for
hypoglycemic effects.
Sudachi is a citrus fruit grows in Tokushima prefecture region
of Japan, and we found a suppressing effect on blood sugar
level elevation by administration of its peel powder in human
obese subjects. Because Sudachi has unique fragrance, we
conducted a study to reveal the aromatic effects of essential oil
from sudachi on the metabolic regulation.
Method: These experiments were worked using male ddY
mice. We exposed vaporized oil of sudachi peal to 7 –8 weeks
old mice for three weeks under the fixed concentration, then
body weight, food intake and water intake were measured. The
intraperitoneal glucose tolerance test (IPGTT) was performed
and the organ weight and plasma lipid levels were evaluated.
We also analyzed expression levels of mRNA related to energy
consumption. In addition, guanethidine was injected to
another group to investigate involvement of the sympathetic
nerve.
Result: We foundweight loss involvingWAT reductionwithout
change in food intake. Triglyceride (TG) in the blood was
reduced by the treatment, and glucose tolerance was also
improved. The expression of hepatic PGC-1 tended to be
increased compared to control. When we administered
guanethidine to the sudachi treated group, the reductions of
plasma TG and WAT weight were canceled.
These data suggest that vaporized sudachi oil may stimulate
olfactory, resulting in the activation of the sympathetic nerve
which participates in the reduction of WAT by facilitation of
the energy consumption.
PC-15
Construct an IDNT-NCP computer program for clinical
management of patients with Diabetes Mellitus
Wen-Ching TEH1, Te-Hsuan LIN1, Hsing-Yi WANG1,
Hui-Chun HSU1, Ling-Wang AN2 , Yu-Hung CHANG1,
Der-Wei HWU1, Yau-Jiunn LEE1*. 1Lee’s Endocrinology Clinic,
Taiwan; 2Beijing Ruijing Diabetes Hospital, China
Objective: Diabetic management model has been markedly
changed over the past few decades especially on dietetic
practice which have prompted dietitians to become more
accountable and aware of treatment outcome and evaluation.
Published evidence has demonstrated the numerous benefits
which can be gained by application of a nutrition care process
(NCP) and standardized language. However, a convenient,
computerized, comprehensive tool to assess key NCP proced-
ure is still lacking.
Materials and methods: A Chinese version of computerized
International Dietetics & Nutrition Terminology (IDNT)
NCP program was constructed to assess diabetes nutrition
behaviors.
Results: The system is built using a Microsoft Solution based
Web Site project template, SQL Server, and jQuery
User Experience technique in rule engine. The trained
Dietitians could be easily download automatically or enter
patient’s basic personal, anthropometric data, physical exam
findings, biochemical data, and food/nutrition history into the
program to complete the nutritional assessment. After com-
pleteness of nutrition assessment, the program will make
inference to the rule base and make nutrition diagnosis.
Dietetics professionals could then make the final diagnosis
decision for the patient based on the diagnosis report gene-
rated by the web based nutrition diagnosis expert system,
which involves identifying and labeling nutrition-related
problems, etiology, signs and symptoms statements for con-
ditions. The systemwill also set up a window for the dietitians
to implement the nutrition intervention, monitoring and
evaluation plan, which involves formulating goals and deter-
mining plans of action and are integrated into overall disease
management and implementation.
The content validity index was calculated by experts’ ratings of
item relevance. The ICVI was 0.7–1.0. To understand the inter-
rater reliability of system, three groups including CDEs and 5
patients, involved the process of inter-rater reliability assess-
ment to test the rating consistency among observational
ratings is 95-0.98.
Conclusion: A Chinese version of IDNT-NCP system build in a
computer program may be a precise, convenient computer-
based system to evaluate and follow the changes of eating
behavior in patients with DM.
Poster Presentations / Diabetes Research and Clinical Practice 120S1 (2016) S65–S211 S90