Diabetes Research and Clinical Practice - Volume 120 - Supplement 1 - October 2016

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Methods: This 1-year follow-up study included 34 obese T2D
individuals (18 males, 16 females) who underwent RYGB in our
hospital. Anthropometric results, glucose levels, lipid profiles,
and serum betatrophin levels were determined before and 1
year after RYGB.
Results: The serum betatrophin level decreased significantly
after RYGB [72.0 (33.4 –180.9) vs. 35.7 (14.8–103.3) ng/mL;
P < 0.001]. The change in betatrophin was significantly posi-
tively correlated with the changes in haemoglobin A1c (A1C)
and fasting plasma glucose (FPG) and negatively correlated
with the changes in the 2-h C-peptide/fasting C-peptide (2hCP/
FCP) and HOMA of beta cell function (HOMA-β%) (P < 0.05).
Multiple stepwise regression analysis showed that the change
in the serum betatrophin level was independently and
significantly associated with the changes in FPG (β = 0.586,
P < 0.001) and 2hCP/FCP (β = –0.309, P = 0.021).
Conclusions: Circulating betatrophin might be involved in the
regulation of glucose control and insulin secretion in obese
Chinese with T2D soon after RYGB.
PI-12
Relationship between gestational weight gain and pregnancy
complications, delivery outcome
Wenjia YANG 1, Feifei HAN1, Xiaoling CAI1, Xueyao HAN1,
Linong JI 1*. 1Peking University People’s University, China
The aim of this study is to analyze the relationship between
gestational weight gain and delivery outcomes, pregnancy
complications.
This retrospective study included 1,102 pregnant women. Data
were extracted from electronic medical records. Baseline
weight and weight change during the whole pregnancy were
recorded. For the statistical analysis, these participants were
divided into four groups based on the weight gain quartiles in
different trimester of pregnancy.
Weight gain in the second trimester (9.1 ± 3.0 kg) of pregnancy
was positively correlated with macrosomia, and negatively
correlated with neonatal death, which is an independent risk
factor of postpartum hemorrhage (OR = 1.067, 95% CI 1.002–
1.137, P = 0.04) andmacrosomia (OR = 1.145, 95% CI 1.027 –1.276,
P = 0.02). Weight gain in the third trimester (6.1 ± 2.6 kg) was
positively correlated with neonate weight and macrosomia,
and negatively correlated with neonatal death, preterm birth,
gestational diabetes, infant of low-birth weight. It is an
independent risk factor of preterm birth (OR = 0.770, 95% CI
0.646–0.916, P < 0.01), infant of low-birth weight (OR = 0.813,
95% CI 0.668 –0.990, P = 0.03) and gestational diabetes (OR =
0.828, 95% CI 0.743 –0.923, P < 0.01). Total gestational weight
gain (15.3 ± 5.2 kg) was positively correlated with neonate
weight and the morbidity of macrosomia, and negatively
correlated with the neonatal death, gestational diabetes,
preterm birth and infant of low-birth weight, which is an
independent risk factor for the development of neonatal death
(OR = 1.063, 95% CI 1.019 –1.109, P < 0.01), postpartum hemor-
rhage (OR = 1.096, 95% CI 1.020–1.178, P = 0.02), macrosomia
(OR = 0.890, 95% CI 0.793 –0.998, P = 0.05), infant of low-birth
weight and gestational diabetes (OR = 0.922, 95% CI 0.865–
0.982, P = 0.01).
According to this analysis, the weight gain during pregnancy
was associated with the gestational complications, adverse
pregnancy outcomes, status of neonatal abnormality in
varying degrees.
PI-15
Assessment of the Dynamic Insulin Secretion and Sensitivity
Test (DISST) in morbidly obese subjects pre and post gastric
bypass
John WILSON 1*, Paul DOCHERTY2, Richard STUBBS3,
Brian CORLEY 1, Jeremy KREBS1. 1University of Otago (Wellington),
2 Biomedical Engineering Department, University of Canterbury,
3 Wakefield Obesity Clinic, Wellington, New Zealand
Recently a new test for the assessment of insulin resistance,
the Dynamic Insulin Sensitivity and Secretion Test (DISST)
[1,2], has been developed as a suitable substitute for the
current but complex reference method, the euglycaemic
hyperinsulinaemic clamp (EIC). The DISST method has been
validated against the clamp in a range of stable state situations
but not after an interventionwhich is known to fundamentally
change glucose homeostasis and insulin sensitivity. The
primary aim of this study is to test preliminary performance
of DISST relative to the EIC in a morbidly obese cohort before
and after bariatric surgery.
Methods: The DISST test was compared to the EIC in pre
surgery morbidly obese patients and at 3 weeks post gastric
bypass surgery. Correlation and Bland-Altman analysis was
performed.
Results: A total of 11 subjects were recruitedwith 10 having pre
surgery and 8 having post surgery DISST and EIC data. Matched
data for all DISST versus EIC showed good correlation between
the two (r = 0.76, CI 0.45–0.90, n = 18). Bland-Altman analysis
showed DISST underestimated the clamp by 0.96 × 10−2 mg L
kg−1 min−1 pmol−1 (95% confidence intervals − 2.24 to 0.32). For
just the pre surgery group correlation between DISST and EIC
was r = 0.81(95% CI 0.37–0.95, n = 10) and with Bland-Altman
analysis DISST again under estimates the clamp by 1.16 × 10−2
mg L kg−1 pmol−1 (95% confidence intervals −2.65 to 0.33). In
the post surgery group correlation between DISST and EIC was
only r = 0.47 (95% CI 0–0.88, n = 8) and Bland Altman analysis
DISST underestimated the clamp by 0.71 × 10−2 mg L kg−1
pmol−1 (95% confidence intervals −1.61 to 0.19).
Conclusion: The DISST test shows promise for use in morbidly
obese individuals compared to the EIC but numbers are small
with wide confidence intervals. Further investigations on
utility especially in the post surgery setting are needed.
References
1. Lotz TF, Chase JG, McAuley KA, Lee DS, Lin J, Hann CE,
Mann JI. Transient and steady-state euglycemic clamp
validation of a model for glycemic control and insulin
sensitivity testing. Diabetes Technol. Ther., 8 (3) (2006), 338–
346.
2. McAuley KA, Berkeley JE, Docherty PD, Lotz TF, Temorenga
LA, Shaw GM,Williams SM, Chase JG,Mann JI. TheDynamic
insulin sensitivity and secretion test-a novel measure of
insulin sensitivity. Metabolism 60 (2011) 1748– 1756.
PI-16
Correlation between uric acid and body fat distribution in type
2 diabetes mellitus
Jung Kyu PARK1, Jae Wan KWON1, Eon Ju JEON1, Eui Dal JUNG1,
Ho Sang SHON1, Ji Hyun LEE1*. 1Department of Internal Medicine,
Catholic University of Daegu, School of Medicine, Korea
There has been a renewed interested in hyperuricemia and its
association with a number of clinical disorders other than
gout, including hypertension, cardiovascular disease, meta-
bolic abnormalities such as obesity and metabolic syndrome.
The relationship between the regional distribution of body fat
and uric acid in patient with type 2 diabetes mellitus (DM) is
not well established. The aim of this study was to investigate
the relationship between uric acid and body fat distribution in
patient with type 2 DM. A total of 228 subjects with type 2 DM
(184 normouricemia and 44 hyperuricemia) were included in
this study. Hyperuricemia was defined >5.7 mg/dL. Clinical
and anthropometric profile, such as body mass index (BMI),
waist and hip circumferences, waist-to-hip ratio, skinfold
thickness, and lipid profiles were measured. Abdominal fat
amount was measured by single slice abdominal computed
tomography scanning. Hyperuricemic group compared with
normouricemic group had statistically increased visceral fat
(132.06 ± 42.58 vs 111.39 ± 42.58 cm2, p = 0.004) amount and
triglyceride (173.41 ± 73.59 vs 130.53 ± 69.41 cm2, p < 0.001). Uric
acid levels were positively correlated with visceral fat amount
Poster Presentations / Diabetes Research and Clinical Practice 120S1 (2016) S65–S211 S186