Diabetes Research and Clinical Practice - Volume 120 - Supplement 1

Results:

A total of 985 was initially identified (Pubmed = 420,

Web of Science = 546, CENTRAL = 19). After removed duplicate

articles (n = 292) and irrelevant articles (n = 669), there were 24

articles selected for full text review and 11 articles left to be

included in present analysis. Overall, patients who are

diagnosed of OSA had a higher chance of CKD with pooled

odds ratio of 2.01 (95% C.I: 1.61

–

2.2); however, the risk was

increased with pooled odds ratio of 2.46 (95% C.I: 2.0

–

3.03) in

patients with diabetes mellitus. In addition, we found that

OSA was consistently associated with a higher proteinuria/

albuminuria and a worse renal function with pooled odds ratio

of 2.44 (95% C.I: 2.12

–

2.80), 1.50 (95% C.I: 1.21

–

1.86) respectively.

Conclusion:

Our report demonstrated that OSA is significantly

associated with CKD, which may be particular important to

patients with diabetes mellitus.

PJ-49

The development of human recombinant protein, GAPtin, for

diabetes

Shih Ting TSENG

1,2

*, Chia Shin YANG

, Yeh CHEN

Department of Endocrinology and Metabolism, Kuang Tien General

Hospital,

Department of Food and Nutrition, Providence University,

Department of Biotechnology, Hungkuang University, Taiwan

Obesity has become a growing epidemic that plaques people of

different cultures worldwide. Obesity is such a major health

concern because it leads to several fatal diseases. Obesity

sufferers are prone to cardiovascular problems, diabetes,

stroke and heart attack. To study these diseases such as

obesity and diabetes, many reports reveals that some proteins

and/or peptides could reduce the blood-glucose level and

inhibit appetite. The major issues are the half-life is very

short of critical polypeptides molecules, and they were very

sensitive to proteinases and were degraded by these enzymes.

In order to increase the half-life of these proteins, three

polypeptides, Adiponectin, Glucagon-like peptide-1, Peptide

YY, will be selected and genetic fused to form a fusion protein,

GAPtin. The structural and biochemical assays will be per-

formed to confirm the protein folding, protein sensitivities,

and protein functions. In further, we proposed that the

abilities of blood sugar control and appetite inhibition of

these fusion proteins could be elevate by using animal studies.

We hope that these recombinant proteins not only can be

used in reducing blood-glucose levels of diabetes patients but

also can prevent the obesity-related diseases. The air sensi-

tivity of latter groups makes the expression and purification

of such proteins challenging. Here we describe a method

for the purification of the GAPtin protein under conditions.

Our procedure consisted of three steps. Subsequent affinity

chromatography on Ni-column, anion exchange on Q-column,

and gel filtration on Superdex 200. Structure and functionality

tests performed with mass spectrometry and circular dichro-

ism spectroscopy assays approved the success of the purifica-

tion procedure. We have already purified GAPtin protein.

PJ-50

Type 2 diabetes increases the risk for gout in Taiwan

Shih Chueh CHEN

, Kuo Ting SUN

Department of

Endocrinology and Metabolism, Cheng Ching Hospital, Taichung,

Depatment of Dentistry, China Medical University Hospital

Taichung, Taiwan

Through impaired kidney ammoniagenesis and because a low

urine PH, type 2 diabetes may be the main factor of

hyperuricemia and gout formation. It was hypothesized that

type 2 diabetes should favor the formation of gout. Therefore,

in a series of 257,156 patients with type 2 diabetes and

1,078,773 without type 2 diabetes. The proportion of gout

was 13.4% in patients with type 2 diabetes and 11.3% in

patients without type 2 diabetes (Adjusted HR 1.28 (1.23, 1.34),

P < 0.001). Multivariable analysis included age (Adjusted HR

1.45 (1.37, 1.53), P < 0.001), sex (Adjusted HR 2.00 (1.93, 2.08),

P < 0.001), and comorbidities of chronic liver disease and

cirrhosis (Adjusted HR 1.20 (1.15, 1.26), P < 0.01), hypertension

(Adjusted HR 1.86(1.78,1 .94), P < 0.001), hyperlipidemia

(Adjusted HR 1.53(1.46, 1.59), P < 0.001), stroke (Adjusted HR

0.91(0.82, 1.01), P > 0.05), coronary artery disease (Adjusted HR

1.07(1.02, 1.13), P < 0.001), chronic kidney disease (Adjusted

HR 1.44(1.35, 1.53), P < 0.001), and obesity (Adjusted HR 1.09

(0.93, 1.28), P < 0.01). All above variables except stroke had

elevated hazard risk for gout formation significantly. There

were still significant higher risks for gout between patients

with type 2 diabetes and patients without type 2 diabetes after

stratified by age, sex and comorbidity. Stepwise regression

analysis identified type 2 diabetes as a strong factor that was

independently associated with the risk for gout. The occur-

rence of gout was most apparent in obese men and in patients

at the oldest age and hyperlipidemia. The 10-year cummula-

tive incidence of gout for patients with type 2 diabetes

was about 1.6 times that without type 2 diabetes. In

conclusion, in view of the strong association between type 2

diabetes and gout formation, it is proposed that gout may be

added to the conditions that potentially are associated with

insulin resistance. Accordingly, it is suggested that patients

with gout, especially if overweight, should be screened for the

presence of type 2 diabetes or components of the metabolic

syndrome.

PJ-51

Impact of metabolic status on the incidence of psoriasis: A

Korean nationwide cohort study

Eun Sook KIM

, Min Ji KIM

, Sung Dae MOON

Hyuk-Sang KWON

The Catholic University of Korea, Korea

Background:

Growing evidence suggests that obesity is a risk

factor for incident psoriasis. This study was aimed to evaluate

the association of obesity and metabolic status with the

incidence of psoriasis.

Methods:

A total of 418,057 adults were followed-up using a

nationwide prospective cohort study in Korea. Participants

were stratified based on the body mass index categories and

metabolic condition.

Results:

During the follow-up visit, 11,054 (2.6%) cases were

found to have psoriasis. Diabetes, hypertension, hyperlipid-

emia, and obesity were all found to be risk factors for incident

psoriasis. Subjects with the metabolically unhealthy non-

obese phenotype (MUNO; hazard ratio [HR], 1.29; 95% confi-

dence interval [CI], 1.22

–

1.37) and metabolically unhealthy

obese phenotype (MUO; HR, 1.33; 95% CI, 1. 26

–

1.41) had a

significantly higher risk of psoriasis incidence as compared to

those with the metabolically healthy non-obese phenotype,

after adjusting for age, sex, smoking, exercise, and income. The

risk of psoriasis development was found to be high among

those with the MUNO and MUO phenotypes in both sexes and

all age groups.

Conclusions:

The metabolic health status was significantly

associated with an increased risk of psoriasis in both obese

and non-obese individuals. However, further studies are

needed to evaluate whether the control of metabolic para-

meters can lower the incidence of psoriasis.

PJ-52

Extra virgin olive oil does not cause post-prandial endothelial

dysfunction unlike processed butter through protective

mechanisms against oxidative stress

Yuji TAJIRI

*, Kentaro YAMADA

Division of Endocrinology and

Metabolism, Kurume University School of Medicine, Japan

It is well known that Traditional Mediterranean Diet (TMD),

characterized by a high consumption of vegetables, legumes,

grains, fruits, nuts and olive oil, reduces blood pressure and

produces benefits on cardiovascular (CV) risk. Previous reports

demonstrated that the intake of extra virgin olive oil (EVOO),

rich in polyphenol compounds, exerted a protective effect

Poster Presentations / Diabetes Research and Clinical Practice 120S1 (2016) S65

–

S211

S207