Diabetes Research and Clinical Practice - Volume 120 - Supplement 1

In the current study, we attempted to implement in situ eNOS/

NO up-regulation in/around skin wounds in a rat model of

diabetes by using statin-loaded tissue engineering scaffolds

(TESs). Statin-loaded TESs with different drug loading

amounts were prepared by a technique of electrospinning.

The obtained TESs were composed of uniform and randomly

arrayed fibres, with diameters of 527 ± 80, 466 ± 117 and

550 ± 118 nm, and with theoretical statin loading amounts of

1.0%, 5.0% and 10.0%, respectively. During in vitro drug release,

all the TESs exhibited fast release phase in the initial 4

days. The in vitro cell culture experiments showed that the

statin-loaded TESs could restore the high-glucose induced

low cell viability and decreasing NO synthesis capabilities in

human umbilical vein endothelial cells. In the rat model of

diabetes, statin-loaded TESs with different drug loading

amounts were administered on the skin wounds, with blank

TES and natural healing wound as control. With the analysis of

immunohistochemistry and microdialysis, the statin-loaded

TESs exhibited promotive effect on eNOS expression and NO

synthesis in regenerated skin tissues during skin wound

healing. Furthermore, the regenerated skin tissues in groups

with statin-loaded TESs exhibited promoted angiogenesis and

higher skin blood flow value in/around skin wounds, and

showed faster wound closure.

The present study indicated that the controlled release of

statin from TESs may be a promising therapy for diabetic skin

wound.

This work was supported by the National Natural Science

Foundation of China (81303239) and the Specialized Research

Fund for the Traditional Chinese Medicine Clinical Research

Center of China (JDZX2015128).

PE-33

Vegetarian diet is associated with higher SBP and fasting

serum triacylglycerol than omnivorous diet in Taiwanese

type 2 diabetic

Chih-Ming SUN

*, Hui-Chun HSU

, Shi-yu CHEN

Tri Service

General Hospital, Taiwan

Vegetarian diets are thought to be efficacious for management

of type 2 diabetes mellitus (T2DM), and have been shown to

be associated with reduction in the prevalence of T2DM in

cohort studies, however differences in vegetarian diet com-

position could result in diverse physiologic outcomes. The

current study evaluated the metabolic effects of vegetarian,

breakfast vegetarian (animal foods excluded only from the

first meal of the day) and omnivorous dietary patterns in

Taiwanese T2DM adults.

Vegetarians (n = 69), breakfast vegetarians (n = 132), and

omnivorous (n = 207) diabetic adults were recruited through

chart review at a clinic in Taiwan.

Individual demographic, anthropometric, and dietary prefer-

ence data were collected from consenting participants, and

blood glucose, hemoglobin A1c, serum creatinine and lipid

profile were analyzed from 8-hour overnight fasting blood

samples. Differences among groups were determined by Chi-

square and Kruskal-Wallis tests (Statistical Package for the

Social Sciences, version 12.0, IBM).

Compared to omnivores, vegetarians had 5% higher sys-

tolic blood pressure (p = 0.023) and 24% higher fasting

serum triacylglycerol (p = 0.003) (15% higher than the recom-

mended value), and 14% lower high-density lipoprotein

cholesterol (p < 0.001). A dose-response relationship was

observed between extent of vegetarianism and these meta-

bolic parameters among the groups. Additionally, a higher

prevalence of anti-hypertensive medication use was observed

in the vegetarians compared to omnivores (p = 0.003). Age,

body mass index, waist to hip circumference ratio, diastolic

blood pressure, duration of diabetes, fasting blood glucose,

hemoglobin A1C, serum total cholesterol and low-density

lipoprotein cholesterol, and serum creatinine did not vart

significantly with dietary pattern. When data was analyzed by

gender, diet effects were more pronounced for women than

men. Female vegetarians had higher systolic blood pressure

(p = 0.017) and serum triacylglycerol (p = 0.044), and lower

high-density lipoprotein cholesterol (p = 0.001) and hemoglo-

bin A1c (p = 0.034), and had higher prevalence of using anti-

hypertensive medication (p = 0.014) than female omnivores.

Higher systolic blood pressure and fasting serum triacylgly-

cerol, and lower high-density lipoprotein cholesterol were

observed in vegetarian compared to omnivorous type 2

diabetic adults. We hypothesize that selection of a contem-

porary Taiwanese vegetarian diet may result in alterations in

macronutrient distribution, with excessive consumption of

refined carbohydrates attributed to vegetarian convenience

foods, as replacement of dietary protein and fat with refined

carbohydrate has been associated with these observed

metabolic alterations in other populations. Further investiga-

tion is necessary to identify specific dietary components of

Taiwanese vegetarian diets that may be implicated in adverse

metabolic changes.

PE-34

A successfully treated case of extremely hyperglycemic crisis

accompanied with rhabdomyolysis and acute kidney injury

Chia-Luen HUANG

*, Yi-Jen HUNG

, Chieng-Hsing LEE

Division of Metabolism and Endocrinology, Department of Internal

Medicine, Tri-Service General Hospital, National Defense Medical

Center, Taipei, Taiwan

We reported a 37-year-old man who demonstrated extremely

high level of serum glucose (3,355 mg/dL) complicated with

diabetic ketoacidosis and hyperosmolar hyperglycemic state

after excessive consumption of sweetened beverage within

one day. His blood sugar levels were gradually decreased

by aggressive fluid resuscitation and insulin infusion.

However, the laboratory evaluation revealed elevated levels

of creatinine, creatinine kinase and myoglobin on day 2.

Simultaneously, a huge renal stone was found on abdominal

plain film. The diagnosis of rhabdomyolysis, staghorn stone

and acute kidney injury were made based on these laboratory

tests. Interesting, his creatinine kinase concentration contin-

ued to rise and persisted with high levels for one week even

blood glucose, ketoacidosis and renal function were recovered.

Finally, this case was successfully survived after aggressive

treatments. Rhabdomyolysis is an uncommon but under-

estimated complication of hyperglycemic crisis. Hence, phy-

sicians should be alerted to this under detected complication

of hyperglycemic crisis which may lead to acute renal failure

yet can be easily diagnosed by a readily available test

—

the

creatinine kinase level.

PE-35

Comparison of clinical characteristics and outcomes of

hospitalized patients with pneumonia between diabetic

patients and non-diabetic patients in Japan

Yoshinori MASUI

, Akahito SAKO

*, Tadanao HARIGAE

Akiko KAWAGUCHI

, Aki SAKURAI

, Teruhiko SATO

Hidekatsu YANAI

Department of Internal Medicine, Kohnodai

Hospital, National Center for Global Health and Medicine,

Department of Respiratory Medicine, Kohnodai Hospital, National

Center for Global Health and Medicine,

Department of Infectious

Diseases, St. Luke

’

s International Hospital, Japan

Background:

Japan has become a super-aging society, and the

number of patients with pneumonia has been increasing.

Diabetes is associated with an increased susceptibility to

infection and infection-related morbidity and mortality.

However, current evidence showing the association between

diabetes and pneumonia in Asian population is very limited.

Objective:

We aimed to elucidate the influence of diabetes on

clinical characteristics and outcomes of pneumonia.

Poster Presentations / Diabetes Research and Clinical Practice 120S1 (2016) S65

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